ELE BOT-242

ELE BOT-242: MICRO-PROPAGATION TECHNOLOGIES

Credit: 3(1+2)

THEORY:

Introduction, History, Advantages and limitations; Types of cultures (seed, embryo, organ, callus, cell), Stages of micropropagation, Axillary bud proliferation (Shoot tip and meristem culture, bud culture),Organogenesis (callus and direct organ formation), Somatic embryogenesis, cell suspension cultures, Production of secondary metabolites,Somaclonal variation, Cryopreservation

PRACTICALS:

Identification and use of equipments in tissue culture Laboratory, Nutrition media composition, sterilization techniques for media, containers and small instruments, sterilization techniques for explants, Preparation of stocks and working solution, Preparation of working medium, Culturing of explants:Seeds, shoot tip and single node,  Callus induction, Induction of somatic embryos regeneration of whole plants from different explants, Hardening procedures.

Practicals (Experiments):

1) Laboratory organization of Plant Tissue Culture Laboratory

2) Safety Measures in Laboratory

3-5) Sterilization techniques: Common Contaminant in Laboratory, Sterilization of glassware, Working of Laminar air flow cabinet

6-7) Culture Media: Definition, Components of Media, Stock Solution, Working Solution, Sterilization of Media

8-9) Preparation and sterilization of growth regulators/thermolabile compounds

10-11) Preparation of working medium

12-13) Experimentation on determining optimum concentration of growth regulators

14) Sterilization techniques for explants

15-18) Callus induction from different parts of plants

19-22) Regeneration of whole plants from induced callus using different parts of plants

23-24) Induction of somatic embryos

25-26) Experiments of synthetic seeds production and testing storability and germination efficiency

27-32) Direct regeneration into whole plants using bud, node and other tissues