ENTO-364

ENTO-364 : INTRODUCTORY NEMATOLOGY

Credits: 2(1+1)

THEORY:
Introduction: History of Phytonematology, Economic importance, Genera Characteristics of Plant Parasitic Nematodes. Nematode – general morphology and biology, classification of nematodes up to family level with emphasis on groups containing economically important genera. Classification of nematodes by habitat, identification of economically important plant parasitic nematode upto generic level with the’ help of keys and descriptions. Symptoms caused by nematodes with examples, Interaction between plant parasitic nematodes and disease causing fungi, bacteria and viruses. Different methods of nematode management. Cultural methods (crop rotation, fallowing, soil amendments, other land management techniques), Physical methods (soil solarization, hot water treatment) Biological methods. Chemical methods (Fumigants and non fumigants) Resistant varieties. IDM.

PRACTICAL:
Methods of survey — sampling methods, collection of soil and plant samples; Extraction of nematodes from soil and plant tissues following combined Cobb’s Decanting and sieving, Baermann’s funnel technique, counting and estimation of plant parasitic nematodes; Preparation of temporary and permanent mounts; Method of preparation of perennial patterns for identification of species of Meloidogyne Study and identification of most important plant parasitic nematodes with special reference to their characteristics and symptomatology – Meloidogyne, Pratylenchus, Heterodera, Ditylenchulus, Globodera, Tylenchulus, Xiphinema, Radopholus, Rotylenchulus and Helicotylenchus. Experimental techniques used in pathogenicity studies with root knot nematode.

Practical (Experiments):
1) Methods of survey- sampling methods, collection of soil and plant samples.
2) Extraction of nematodes from soil and plant tissues following combined Cobb’s Decanting and sieving and Baermann’s funnel technique.
3) Counting and estimation of plant parasitic nematode.
4) Preparation of temporary and permanent mounts.
5) Method of preparation of perennial patterns for identification of species of Meloidogyne.
6) Study and identification of most important plant parasitic nematodes with special reference to their characteristics and symptomotology- Meloidogyne and Rotylenchulus.
7) Pratylenchus and Helicotylenchus.
8) Heterodera and Xiphinema.
9) Ditylenchus and Globodera.
10) Tylenchulus and Radopholus.
11) Mass Production of EPN.
12) Pathogenicity studies of EPN.
13) Experimental techniques used in pathogenicity studies with root-knot nematode.
14) Soil solarization and sterilization methods.